Effects of S-Adenosylhomocysteine and Homocysteine on DNA Damage and Cell Cytotoxicity in Murine Hepatic and Microglia Cell Lines

doi:10.1002/jbt.20298

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/23131

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/23131

Title:	Effects of S-Adenosylhomocysteine and Homocysteine on DNA Damage and Cell Cytotoxicity in Murine Hepatic and Microglia Cell Lines
Authors:	Chia-Chyuan Liu Wen-Yueh Ho Kuen-Lin Leu Hsin-Mao Tsai Tsai-Hsiu Yang
Contributors:	化粧品應用與管理系
Keywords:	S-adenosylhomocysteine Homocysteine Cytotoxicity DNA Damage DNA Hypomethylation
Date:	2009-09
Issue Date:	2010-10-26 16:06:53 (UTC+8)
Publisher:	John Wiley & Sons Inc
Abstract:	Limited research has been performed on S-adenosylhomocysteine (SAH) or homocysteine (Hcy)-evoked cell damage in hepatic and neuronal cells. In this study, we assessed effects of SAH or Hcy on cell cytotoxicity and DNA damage in hepatic and neuronal cells and attempted to find the underlying mechanism. Cell cytotoxicity and DNA damage were evaluated in murine hepatic cells (BNL CL.2 cell line) and microglia cells (BV-2 cell line) with SAH or Hcy treatment for 48 h. The influences of SAH or Hcy on lipid peroxidation and DNA methylation were also measured in both cell lines. SAH (5–20 μM) or Hcy (1–5 mM) dose dependently inhibited cell cytotoxicity and enhanced DNA damage in both types of cells. Furthermore, SAH treatment markedly increased intracellular SAH levels and DNA hypomethylation, whereas Hcy caused minimal effects on these two parameters at much higher concentrations. Hcy significantly induced lipid peroxidation, but not SAH. The present results show that SAH might cause cellular DNA damage in hepatic and microglia cells by DNA hypomethylation, resulting in irreversible DNA damage and increased cell cytotoxicity. In addition, higher Hcy could induce cellular DNA damage through increased lipid peroxidation and DNA hypomethylation. We suggest that SAH is a better marker of cell damage than Hcy in hepatic and microglia cells.
Relation:	Journal of Biochemical and Molecular Toxicology 23(5)：p.349-356
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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