利用巨噬細胞株模式篩選具免疫機能植物源乳酸菌之研究

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 博碩士論文 > Item 310902800/23106

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標題:	利用巨噬細胞株模式篩選具免疫機能植物源乳酸菌之研究 Screening of lactic acid bacteria isolated from plant resources with immunopotentiating activities on RAW264.7 macrophages
作者:	楊憶華
貢獻者:	常振鎧劉坤湘嘉南藥理科技大學：生物科技系暨研究所
關鍵字:	巨噬細胞植物源乳酸菌免疫機能吞噬能力一氧化氮 immunopotentiating activities lactic acid bacteria isolated from
日期:	2010
上傳時間:	2010-10-12 15:11:26 (UTC+8)
摘要:	本研究以植物源乳酸菌為對象，進行具免疫機能乳酸菌種的篩選。以小鼠巨噬細胞株RAW 264.7為實驗材料，利用巨噬細胞株模式探討植物源乳酸菌激活巨噬細胞之能力，以刺激巨噬細胞株的吞噬能力、誘發巨噬細胞株分泌nitric oxide (NO)、tumor necrosis factor-α (TNF-α) 及interleukin-6 (IL-6) 等做為評估指標。結果顯示，有30株乳酸菌株確實具有刺激巨噬細胞株增加吞噬的能力。其中B0018、B0019、B0020、B0040、B0042、B0044、B0046、B0052、B0088、B0091、B0110、B0145等12株乳酸菌具有激活巨噬細胞株之NO生成能力。 NO已知會影響多種細胞激素包含IL-6及TNF-α分泌。Western blot結果顯示乳酸菌可刺激巨噬細胞產生inducible nitric oxide synthase (iNOS)。其中，B0040、B0046及B0091具有顯著激活小鼠巨噬細胞株RAW264.7 刺激產生 IL-6及TNF-α、釋放NO、增加巨噬細胞吞噬活性。綜合以上結果，植物源乳酸菌確實可增強免疫活性。 The present study aims were to investigate the immunopotentiating activities of lactic acid bacteria isolated from plant resources (LABIPR) in vitro. A murine macrophage cell line RAW264.7 was stimulated with several strains of LABIPR. Phagocytotic assay by neutral red, and the production of nitric oxide (NO) , tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were evaluated the immunopotentiating activities of LABIPR on RAW264.7 cells. The results showed that 30 LABIPR strains could stimulate the phagocytosis of macrophages. The twelve LABIPR strains including B0018, B0019, B0020, B0040, B0042, B0044, B0046, B0052, B0088, B0091, B0110 and B0145, could stimulate the NO production of macrophages. NO was known to affect the secretion of several cytokines containing IL-6 and TNF-α. Some strains of lactic acid bacteria express significant levels of iNOS-like protein as shown by Western blot analysis.Among, this study about the significantly activation of murine macrophage Raw 264.7 cells showed that heat-killed LABIPR B0040,B0046 and B0091 stimulated the production of IL-6 and TNF-α production, nitric oxide release, and increased phagocytic activity in macrophages, express of iNOS-like protein. These results, taken together, indicate that LABIPR might play a critical role in the immunopotentiating activities.
關聯:	校內校外均不公開，學年度：98，127頁
显示于类别:	[生物科技系(所)] 博碩士論文

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