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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/23090


    標題: 虎杖根及檸檬桉樹脂萃取物對金黃色葡萄球菌存活及生物膜形成的影響
    Effects of the extracts from Polygonum cuspidatum root and kino of Eucalyptus citriodora on the viability and biofilm formation of Staphylococcus aureus
    作者: 李世源
    貢獻者: 林翠品
    葉東柏
    嘉南藥理科技大學:生物科技系暨研究所
    關鍵字: 虎杖
    生物膜
    金黃色葡萄球菌
    大黃素
    emodin
    Staphylococcus aureus
    biofilm
    Polygonum cuspidatum root
    日期: 2010
    上傳時間: 2010-10-12 15:10:54 (UTC+8)
    摘要: 金黃色葡萄球菌是造成院內感染的主要細菌,具有生物膜的金黃色葡萄球菌能夠抵擋抗生素、消毒劑及免疫系統的作用,不容易被殺死,會導致心內膜炎、骨髓炎及感染相關的致命疾病。本研究證明虎杖根乙醇萃取物PcE1 濃度 4 mg/ml時能殺死65-70%金黃色葡萄球菌SA113及SA16,2 mg/ml就能殺死金黃色葡萄球菌SA17。而且虎杖根乙醇萃取物的乙酸乙酯分劃物PcE2E,在50 g/ml時分別抑制36.9%、26.7%及15.2%的SA113、SA16及SA17生長。進一步也發現虎杖根乙醇萃取物中的純物質大黃素 (Emodin)在50 g/ml時,分別抑制SA113、SA16及SA17的生長,大黃素抑制率分別為44.8%、14.9%及28.8%。此外,我們也發現虎杖根乙醇萃取物(PcE1) 及其正己烷分劃物(E1HF1、E1HF2及E1F3) 在50 g/ml分別抑制50%、50%、60%及75% SA113生物膜的形成。而PcE2E在50 g/ml 時對 SA113與臨床菌種,SA16及SA17具有明顯抑制生物膜生成的效果,抑制率分別為68%、43.6%及70.6%。虎杖根乙醇萃取物中純物質,大黃素在12.5 g/ml時,對 SA113、SA16及SA17具有明顯抑制生物膜生成的效果,抑制率分別為71.1%、49.6%及75.3%,所以大黃素是虎杖根乙醇萃取物抑制金黃色葡萄球菌生物膜的形成的主要成分。
    Staphylococcus aureus is a leading cause of nosocomial infection. Many of these diseases, including endocarditis, osteomyelitis, and foreign-body related infections, appear to be caused by biofilm-associated S. aureus. Biofilm-associated bacteria show an innate resistance to antibiotics, disinfectants, and clearance by host defenses. This study, found that the ethanolic extract of P. cuspidatum, PcE1 kill Staphylococcus aureus SA113 and SA17 by 60-70% at 4 mg/ml, kill .S. aureus SA16 completely at 2 mg/ml. The ethylacetate fraction of Polygonum cuspidatum, PcE2E at 50 g/ml also inhibit the growth of Staphylococcus aureus SA113, SA16 and SA17 by 36.9%, 26.7% and 15.2%, respectively. The main compound in the ethanolic extract of Polygonum cuspidatum, emodin inhibit the growth by S. aureus SA113, SA16 and SA17 by 44.8%、14.9% and 28.8%, respectively. Moreover, Results showed that the ethanolic extract of P. cuspidatum, PcE1and its ethyl acetate fraction, E1HF1, E1HF2 and E1HF3 at 50 g/ml inhibit the biofilm formation of S. aureus SA113 by 50%、50%、60% and 75%, respectively. The ethyl acetate fraction of P. cuspidatum, PcE2E at 50 g/ml also inhibits the biofilm formation by S. aureus SA113, SA16 and SA17 by 68%、43.6% and 70.6%, respectively. The main compound in the ethanolic extract of P. cuspidatum, emodin at 12.5 g/ml inhibit the biofilm formation of S. aureus SA113, SA16 and SA17 by 71.1%、49.6% and 75.3%, respectively. These results suggest that emodin is a key compound in the ethanolic extract of P. cuspidatum, which inhibits the growth and biofilm formation by S. aureus.
    關聯: 校內校外均不公開,學年度:98,54頁
    Appears in Collections:[生物科技系(所)] 博碩士論文

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