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    標題: 米醋醱酵液的萃取物在化粧品原料功效上的研究
    Study on the function of cosmetic materials from ferment liquid extracts of the rice vinegar
    作者: 楊姍燁
    貢獻者: 呂昆霖
    嘉南藥理科技大學:化妝品科技研究所
    關鍵字: 米醋
    抗菌
    抗氧化
    抗老化
    保濕
    醱酵
    moisturizing
    anti-aging
    antibacteria
    antioxidation
    Rice Vinegar
    fermentation
    日期: 2010
    上傳時間: 2010-10-08 13:42:11 (UTC+8)
    摘要: 本研究目的是以釀造米醋之餘,所剩的醱酵液體當作材料,希望透過微生物的醱酵技術,當作化妝品原料功效上的開發。本實驗作各種活性詴驗,結果如下:
    (1) 在抑制基質金屬蛋白酶(MMP)方面,樣品總濃度在1g/mL時,於作用於24小時後,具有抑制MMP (約72kDa)的酵素活性功效。
    (2) 在抑菌方面之紙錠擴散詴驗,樣品總濃度在1g/mL時,其結果顯示對於枯草桿菌(抑菌圈11mm)、金黃色葡萄球菌抑菌圈(10.8mm)有抑制效果,但對於大腸桿菌、綠膿桿菌、白色念珠菌則沒有抑菌效果。
    (3) 在保濕能力測定方面,分別做了四種相對濕度,包括在90%、77%、55%、33%相對濕度時,樣品的吸濕實驗和保水能力實驗,結果顯示醋之醱酵液體,總濃度1g/mL時,在相對濕度90%時,在24小時可增加1.75%,有良好的吸濕效果,而在相對濕度77%時,在24小時可增加0.25%,吸濕能力次之。但在保水能力效果均不彰。
    (4) 在抗氧化方面,做了五種抗氧化實驗,有清除DPPH自由基、TEAC總抗氧化力、抑制脂質過氧化能力、還原能力、螯合亞鐵離子能力,發現米醋醱酵液濃度為10mg/mL時,可清除80% DPPH自由基,而濃度為1.4mg/mL時相當於33.12μM Trolox 的抗氧化能力濃度為0.5mg/mL時,還原能力的OD700 值為0.373,算是有些效果,但濃度為1mg/mL時,對螯合亞鐵離子的效果只有9.7%。
    以整體看來,綜合以上的實驗結果,醋之醱酵液體,可做為化妝品新原料的開發,且具有保濕、抑制基質金屬蛋白酶活性、抗氧化的功效。
    This study used the ferment liquid of rice vinegar as a material to develop the potential efficiency of cosmetic materials. The results are shown as the following:
    1. To treat the MMPs with the sample that concentration
    was in 1g/mL. It revealed the great inhibition of MMPs
    activity(MW about 72kDa) after 24 hours.
    2. Paper disc diffusion tests in microorganisms, we found that the concentration of sample was in 1g/mL, it was efficent to the inhibit the growth of B. subtilis (inhibitory zone 11mm) and Staphylococcus aureus bacteria (10.8mm), then no inhibitory ability in Escherichia coli, Pseudomonas aeruginosa, and Candida albicans.
    3. To determinate the ability of keeping moisture, the experiments of moisture absorption and water retention were proceeded at 33%, 55%, 77%, and 90% relative humidity, respectively. The results showed that the ferment liquid of rice vinegar could increase 1.75% of moisture absorption rate on 24 hour at 90% relative humidity that the concentration of ferment liquid was in 1g/mL. The ferment liquid also enhanced 0.25% of moisture absorption rate on 24 hour at 77% relative humidity. However, function of water retention in this study was not found.
    4. In anti-oxidation, this study showed five results of scavenging free radical including the DPPH, total anti-oxidant capacity of TEAC, inhibition of lipid peroxidation, reducing power, and ferrous ion chelating ability. We found that the concentration of ferment liquid of rice vinegar was in 10mg/mL showing 80% eliminating rate on DPPH free radical and the concentration of the sample was in 1.4mg/mL equivalent of 33.12μM total anti-oxidant capacity of TEAC then it displayed only 9.7% for chelating of ferrous ion that the concentration of the sample was in 1mg/mL.
    According to the experimental results, the ferment liquid of rice vinegar can be used as the new materials of cosmetic science. It
    exhibits the function of moisture absorption, the inhibitory of
    matrix metalloproteinase and anti-oxidative effect.
    Appears in Collections:[化妝品應用與管理系(所)] 博碩士論文

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