English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18057/20255 (89%)
造訪人次 : 1498046      線上人數 : 722
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/23068


    標題: 中藥鈎藤在化妝品生物活性之研究
    Studies on the cosmetic bioactivities from Uncaria rhynchophylla (Miq.) Jacks.
    作者: 劉逸馨
    貢獻者: 丁秀玉
    嘉南藥理科技大學:化妝品科技研究所
    關鍵字: DPPH自由基
    B16黑色素細胞
    鈎藤
    中文關鍵字
    Uncaria rhynchophylla
    antimelanogensis
    antioxidation
    日期: 2010
    上傳時間: 2010-10-08 13:41:51 (UTC+8)
    摘要: 中藥鈎藤Uncaria rhynchophylla (Miq.) Jacks.的帶鈎枝條以乙醇進性抽提,抽提物經分配萃取共得到正己烷層、甲醇層、正丁醇層及水層,進行有效成分分離及其在化妝品方面之生物活性試驗,共得到15個化合物。Triterpenoid類: Ursolic acid (1) 和Oleanolic acid (2); Flavonoid類: (-)-Epicatechin (3) 和Quercetin (4); Phenolics 類: Syringic acid (5)、Caffeic acid (6)、Protocatechuic acid (7)和Vanillic acid (8); Phenolic acid ester類: Methyl chlorogenate (9); Coumarin類: Scopoletin (10); Sterol類: β-Sitosteryl-β-D-glucoside (11)和β-Sitosterol (13)及Aliphatic類: Methyl hexadecanoate (12)、Eicosanoic acid (14)和Stearyl stearate (15)。上述化合物經各種光譜分析加以證明及確認其化學結構式,化合物2、6、7、8、9、12、14 及 15為首次從鈎藤純化分離得到之成分。
    從鈎藤純化分離得到之具有降低B16黑色素細胞內黑色素含量生物活性的化合物為(-)-Epicatechin (3)、Quercetin (4)、Caffeic acid (6)、Methyl chlorogenate (9)、β-Sitosteryl-β-D-glucoside (11)及β-Sitosterol (13) 其中以化合物3及4濃度分別為100 µg/mL 及 25µg/mL降低黑色素含量效果最為顯著,其含量率分別為30.39 % 及 9.93 %。另外,具有清除DPPH自由基生物活性的化合物為(-)-Epicatechin (3)、Quercetin (4)、Syringic acid (5)、Caffeic acid (6)、Protocatechuic acid (7) 及Methyl chlorogenate (9)清除DPPH自由基活性(SC50值分別為4.03、2.10、1.68、2.17、1.87及2.92 μg/mL)均比L-Ascorbic acid (SC50值為5.65 μg/mL)強。
    The antioxidation and antimelanogensis activities of Uncaria rhynchophylla-derived materials were evaluated. The ethanol extract of U. rhynchophylla was partitioned into n-hexane, methanol, BuOH, and water. The n-hexane and methanol soluble partition exhibited antioxidation and antimelanogensis activities, and further purified by CC, Sephadex LH-20, Lobar RP-8, and HPLC. The bioassay guided purification to get fifteen compounds: ursolic acid (1), oleanolic acid (2), (-)-epicatechin (3), quercetin(4), syringic acid (5), caffeic acid (6), protocatechuic acid (7), vanillic acid (8), methyl chlorogenate (9), scopoletin(10), β-sitosteryl-β-D-glucoside (11), β-sitosterol (13), Methyl hexadecanoate (12), eicosanoic acid (14), and Stearyl stearate (15) from U. rhynchophylla. Of these fifteen compounds, 2, 6, 7, 8, 9, 12, 14, and 15 are identified for the first time from U. rhynchophylla. Six isolated compounds showed inhibitory activity melanin content of melanoma B16 cells of (-)-epicatechin (3), quercetin (4), caffeic acid (6), methyl chlorogenate (9), β-sitosteryl-β-D-glucoside (11), and β-sitosterol (13). Compounds 3 and 4 showed potent antimelanogensis activity with an concent value of 30.39 and 9.93 %. The compounds (-)-epicatechin (3), quercetin (4), syringic acid (5), caffeic acid (6), protocatechuic acid (7), methyl chlorogenate (9) showed DPPH free radical scavenging activity with the SC50 values of 4.03, 2.10, 1.68, 2.17, 1.87, and 2.92 μg/mL, respectively. All of them exhibited higher activity than ascorbic acid (5.65 μg/mL).
    關聯: 校內校外均不公開,學年度:98,95頁
    顯示於類別:[化妝品應用與管理系(所)] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML1585檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋