海藻脂氧合酶催化產物之抗菌性

CNU IR > Chna Nan Annual Bulletin > Vol.22 (1996) > Item 310902800/23025

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Title:	海藻脂氧合酶催化產物之抗菌性 Antimicrobial Activity of Sea Algal Lipoxygenase-Catalyzed Oxidation Products of Fatty Acids
Authors:	郭建民吳鴻程黃慶華葉東柏
Contributors:	食品衛生系
Keywords:	海藻脂氧合酶抗菌作用 sea algae lipoxygenase antimicrobial activity Enteromorpha intestinalis
Date:	1996
Issue Date:	2010-09-17 15:03:04 (UTC+8)
Abstract:	本研究發現之海藻(Enteromorpha intestinalis)脂氧合酶催化脂肪酸產物對食物中毒原因菌Vibrio parahaemolyticcus與Staphyococcus、腐敗菌Bacillus subtilis、水產病原菌Aeromonas hydrophila 與Staphylococcus pyogenes具有抑菌作用，但對Enterobacter aerogenes, Pseudomonas aeruginosa, Saccharomyces cerevisiae, Salmonella」entenbe等微生物則無影響。此為添加海藻於食品中，具有延長貯藏期限之可能原因。分析海藻脂氧合酶催化18 :2之產物為9-及11 3-HODE (hydroperoxyoctadecadienoic acid)，比例為66.3: 33.7。脂氧合酶催化18 :3之產物為13-HOTE (hydroperoxyoctadecatrienoic acid)。海藻脂氧合酶催化20:4之產物為12-及15-HETE(hydroxyeicosatetraenoic acid)，比例為73.7: 26.3。海藻脂氧合酶催化產物之抗菌性，可能來自所形成之過氧化物。 The sea algae (Enteromorpha intesinalis) Upoxygenase pOXJ-catalyzd oxidation products of linoleic acid were assayed for aotimicrobial activity. The LOX-catalyzed products were found having andmicrobiad activity to Lactobacilius plantuum, Vibrio parahaemolyticcus, Bacilhs subfiis, Aeromonas hydrophila, Staphylococcus pyogenes, and Staphylococcus aureus wbile no antimicrobial activity to Enterobacter auogenes, Pseudomonas aeruginosa, Seccharomyces cerevisiae, and Salmonelhz sentenberg. Beased on retention time in normal or revem phase HPLC analym, products derived from linoleic acid treated with sea algal LOX were 9-and 13-HODE (hydroperoxyoctadecadienoic acid) with a ratio of 66.3: 33.7. The product of linolenic acid reacted with algal LOX was 13-HOTE (hydroperoxyoctadecatrienoic acid). The products of sea algal LOx treated with arachidonoic acid were 12-and 15-HETE (hydroxyeicosatetraenoic acid) with a ratio of 73.7: 26.3.
Relation:	嘉南學報 22期：p.163-170
Appears in Collections:	[Chna Nan Annual Bulletin] Vol.22 (1996) [Dept. of Food Science & Technology] Periodical Articles

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