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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22932


    標題: 葉綠素a對3T3-L1前趨脂肪細胞之抗肥胖活性及分子機制作用研究
    Study on antiobesity activity of chlororophylla and its molecular mechanism (s) of action in 3T3-L1 adipocytes
    作者: 黃于珍
    貢獻者: 吳淑靜
    嘉南藥理科技大學:藥物科技研究所
    關鍵字: 水丁香
    葉綠素a
    細胞凋亡
    Bcl-2
    caspase-3
    AMPK
    Bax
    CD95
    chlorophyll a
    Ludwigia octovalvis
    日期: 2009
    上傳時間: 2010-06-09 09:34:30 (UTC+8)
    摘要: 水丁香Ludwigia octovalvi s (柳葉菜科)為一種水生植物,廣泛分佈於台灣的沼澤地區。在民間傳統上,它用於治療水腫、抗肝炎及降血壓。而對 3T3-L1前驅脂肪細胞,經系統性的活性追蹤,我們證實從水丁香的葉部所分離穫得之化合物〝葉綠素a(CHL-a)〞實驗顯示具有優異的抗增生活性。對3T3-L1細胞之凋亡路徑會造成影響其IC50值為最低。
    本研究,我們再深入探究CHL-a對3T3L-1細胞之細胞凋亡分子機制,發現在5-30 nM,CHL-a顯示對sub-G峰呈劑量-依賴性蓄積作用並且細胞週期停滯於G0/G1期。經48小時處理後,30 nM CHL-a會降低細胞存活率,誘導DNA斷裂片的出現及增加caspase-3的活性。以西方墨點分析結果顯示CHL-a會降低Bcl-2之表現,並且提高Bax及CD95 (APO-1/CD95)之表現呈-時間及劑量依賴性效應。然而,CHL-a會提昇AMPK及P-AMPK之表現,調降PPAR-γ的表現。綜合上述研究結果證實CHL-a能誘導脂肪細胞凋亡,主要機制是透過CD95系統,調控Bcl-2家族蛋白質及活化caspase-3所致,並活化AMPK訊息路徑展現抗脂肪合成之作用。
    Ludwigia octovalvis (Jacq.) P. H. Raven (Onagraceae) is an aquatic plant widely distributed in the wet area of Taiwan. It is used as a traditional treatment for edema, nephritis, and hypertension. The results showed that ethanol extract of L. octovalvis (EELO) possessed the lowest IC50 value against the 3T3-L1 cells. Fraction 3 was found to induce significant cell death in 3T3-L1 cells. We investigated the effect of chlorophyll a (CHL-a) isolated from the leaves of L. octovalvis on the apoptotic pathway in 3T3-L1 cells. The results showed that CHL-a was the most effective with an IC50 value at 48 h. At concentration of 5~30 nM, CHL-a exhibited a dose-dependent accumulation of the Sub-G1 peak and caused G0/G1-phase arrest. After 48 h treatment, 30 nM CHL-a reduced the cell viability, induced the appearance of DNA fragments, and increased the activity of the caspase-3. Western blot data revealed that CHL-a decreased level of Bcl-2, and increased the expression of Bax and CD95 (APO-1/CD95) in a time- and a dose-dependent manner. Furthermore, CHL-a exposure up-regulated the levels of AMPK and P-AMPK, but the expression of PPAR-γ was down-regulated. These data indicate that CHL-a induced apoptosis of mature adipocytes through mediating the CD95 (APO-1/CD95) system, and modulation of Bcl-2 family proteins and caspase-3 activity, as well as exerting anti-adipogenesis ability by activating the AMPK signaling pathway.
    關聯: 校內一年後公開,校外永不公開,學年度:97, 38 頁
    Appears in Collections:[藥學系(所)] 博碩士論文

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