同半胱胺酸對大鼠熱中風之影響

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標題:	同半胱胺酸對大鼠熱中風之影響 Effects of treatment with homocysteine on heatstroke in rats
作者:	柯玟君
貢獻者:	楊彩秀劉家全嘉南藥理科技大學：營養與保健科技研究所
關鍵字:	熱中風同半胱胺酸神經損傷細胞介質素脂質過氧化 heatstroke homocysteine neuronal damage cytokines lipid peroxidation
日期:	2009
上傳時間:	2010-04-15 16:47:26 (UTC+8)
摘要:	中文摘要許多研究指出，血中高濃度同半胱胺酸(Hcy)會促進神經損傷或死亡，同時也是心血管疾病重要的危險因子之一。熱中風多發生於高溫高濕的環境下的工作者，熱中風患者及動物體皆有中樞缺血、缺氧及神經損傷等病理現象，致死率極高。有文獻指出，心血管疾病患者較容易發生熱中風。然而，鮮少有報告討論Hcy在熱中風生成中扮演何種角色，是否血中高濃度同半胱胺酸也可能是熱中風的危險因子；再者，目前並無任何策略可完全改善熱中風誘發的各種損傷，因此熱中風的預防格外重要。本實驗主要藉由大鼠熱中風動物模式來探討Hcy是否對熱中風的生成及所致的傷害有所影響。大鼠麻醉後，於42℃環境溫度(Ta)下誘發熱中風生成，誘發期間分別連續灌流注射(continuous infusion)高濃度Hcy或0.9％生理食鹽水觀察影響情況。結果顯示，連續注射高濃度Hcy會使得誘發熱中風生成時間縮短；心跳速率(HR)及直腸溫度(Tco)隨著誘發時間的增加而顯著升高。而大鼠在連續注射高濃度同半胱胺酸組，於熱中風所致缺血、缺氧及神經損傷情況也顯著提前表現出。在熱中風生成過程中，我們也觀察連續灌流注射高濃度Hcy對大鼠體內自由基與細胞介質素的影響，結果發現，連續灌流注射高濃度Hcy的熱中風大鼠會提早表現出血漿中高濃度自由基與細胞介質素的情形，同時也伴隨有組織DNA傷害。因此根據我們實驗結果推論，血中具高濃度同半胱胺酸的動物體確實可能也是熱中風生成的可能高危險因子，而使動物體較易被誘發生成熱中風，同時也會提前表現出熱中風導致的傷害。關鍵字：熱中風、同半胱胺酸、神經損傷、細胞介質素、脂質過氧化 Abstract Many studies indicated that plasma high levels of homocysteine(Hcy) might enhance occurrence of neuronal injury or death, and it could be also one of the high risk factor in cardiovascular disease. Workers in high temperature and humidity were easy to induce heatstroke formation. Studies found that the heatstroke patients and animals all displayed the central ischemia, hypoxia, neuronal damage, and high mortality. Many literatures suggested that the patients of cardiovascular diseases might be easier to evoke heatstroke induction. However, there is less attention to investigate what role of the Hcy played during heatstroke induction, and whether plasma high levels of Hcy could be also one of high risk factor in heatstroke induction. So far, there is no one strategy can be to tall therapy the heatstroke- induced damage. Consequently, it seems more important to prevent the heatstroke induction. The main purpose of this study is to observe the effects of Hcy on heatstroke induction and heatstroke- induced damage by a rat model. Rats under anedthesia were exposed to an ambient temperature(42℃) to induced heatstroke. We observe effects of continuous infusion with high concentration Hcy(17.5 g/min, i.v.) or 0.9％ normal saline(50 l/min, i.v.) on heatstroke. Our results showed that rats with continuous infusion high concentration Hcy were significantly shrunk the duration time of heatstroke induction, but raised the beats of heart rate and colonic temperature. “The situations of heatstroke- induced cerebral ischemia, hypoxia and neuronal damage” were apparently revealed ahead of time in rats with continuous infusion Hcy group. During heatstroke induction, we also observe the effects of continuous infusion high concentration Hcy on levels of free radicals and cytokines in rats. The similar results were also obtained, the plasma levels of free radicals and cytokines were significantly expressed in advance in rats of high concentration Hcy group. Meanwhile, the DNA damage of tissue was also obtained ahead of time high concentration Hcy during heatstroke. According to our results, we speculated that animals with high levels of Hcy may probably be one of high risk factors to early induce heatstroke formation, and beforehand reveal or aggravate the heatstroke- induced damage.
關聯:	校內公開，校外永不公開，學年度：97，53 頁
顯示於類別:	[保健營養系(所) ] 博碩士論文

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