English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17326/19632 (88%)
Visitors : 3482477      Online Users : 47
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22287

    標題: Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression
    作者: Shu-Lin Chang
    Yi-Ming Chiang
    Cicero Lee-Tian Chang
    Hsu-Hua Yeh
    Lie-Fen Shyur
    Yueh-Hsiung Kuo
    Tung-Kung Wu
    Wen-Chin Yang
    貢獻者: 藥物科技研究所
    關鍵字: Centaurein
    Interferon gamma
    Bidens pilosa
    日期: 2007-06
    上傳時間: 2010-01-15 14:39:47 (UTC+8)
    出版者: Elsevier
    摘要: Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-γ promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-γ promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-γ promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 μg/ml) and its aglycone, centaureidin (EC50 = 0.9 μg/ml), isolated from this butanol subfraction, augmented IFN-γ promoter activity by four-fold. Consistent with the role of centaurein or its aglycone in IFN-γ regulation, we showed that centaurein induced the activity of NFAT and NFκB enhancers, located within the IFN-γ promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-γ transcription, probably via NFAT and NFκB in T cells.
    Appears in Collections:[藥學系(所)] 期刊論文

    Files in This Item:

    File SizeFormat

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback