Characterization of a planar l-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane

doi:10.1016/j.snb.2006.05.022

CNU IR > Pharmacy and Science > Dept. of Food Science & Technology > Periodical Articles > Item 310902800/22217

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22217

Title:	Characterization of a planar l-glutamate amperometric biosensor immobilized with a photo-crosslinkable polymer membrane
Authors:	Ku-Shang Chang Chen-Kai Chang Su-Fen Chou Hsei-Chen Han Chien-Yuan Chen
Contributors:	食品科技系生物科技系
Keywords:	PVA-SbQ Planar electrode Biosensor Shelf-life l-Glutamate
Date:	2007-03
Issue Date:	2010-01-15 10:18:48 (UTC+8)
Publisher:	Elsevier
Abstract:	A photo-crosslinkable polymer mixed with bovine serum albumin was adapted to fabricate an amperometric planar l-glutamate biosensor. The characteristics of this amperometric biosensor during aging of the membrane, its reproducibility and the effects of storage conditions on the long-term stability of the biosensor were studied. The steady-state method (R.S.D. = 3.69%) was more appropriate than the kinetic method (R.S.D. = 7.70%) for measuring the concentration monosodium glutamate. The l-glutamate amperometric biosensor exhibited remarkable long-term stability (150 days) when maintained in a dry dark state. Most of the functional characteristics of the biosensor were restored within 5 min (wet-up times) of repeated wetting and drying. The long-term stability and short wet-up times of the electrode suggests its suitability for use in the design of real time or flow injection biosensors.
Relation:	Sensors and Actuators B: Chemical 122(1)： p.195-203
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Food Science & Technology] Periodical Articles

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