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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22210


    標題: Expression of CDC25B in human spermatozoa.
    作者: Yen-Ni Teng
    Chia-Ling Chung
    Yung-Ming Lin
    Hsien-An Pan
    Rui-Wen Liao
    Pao-Lin Kuo
    貢獻者: 生物科技系
    關鍵字: Human spermatozoa
    CDC25B
    RT-PCR
    immunofluorescence staining
    real-time RT-PCR
    日期: 2007-08
    上傳時間: 2010-01-12 09:13:19 (UTC+8)
    出版者: Elsevier Science Inc
    摘要: Objective: To explore the role of CDC25B protein in postmeiotic germ cells.
    Design: In vitro experiment.
    Setting: University-based reproductive genetics laboratory.
    Patient(s): Fertile and infertile volunteers.
    Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR, and immunostaining for CDC25B.
    Main Outcome Measure: Expression profiling of CDC25B in human spermatozoa.
    Result(s): Four splicing variants (CDC25B1, B2, B3, and B4) are expressed in human spermatozoa. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa, and weak staining in the principal piece and cytosol of the head. The messenger RNA (mRNA) transcript level of CDC25B was increased in sperm samples of men with asthenospermia.
    Conclusion(s): The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells.
    Appears in Collections:[生物科技系(所)] 期刊論文

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