Identification of Phenolic Antioxidants from Sword Brake Fern (Pteris ensiformis Burm.)

doi:10.1016/j.foodchem.2007.03.055

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/22196

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22196

Title:	Identification of Phenolic Antioxidants from Sword Brake Fern (Pteris ensiformis Burm.)
Authors:	Yung-Husan Chen Fang-Rong Chang Yih-Jer Lin Lisu Wang Jinn-Fen Chen Yang-Chang Wu Ming-Jiuan Wu
Contributors:	生物科技系
Keywords:	Pteris ensiformis DPPH Caffeic acid Hispidin TEAC
Date:	2007
Issue Date:	2010-01-12 09:12:58 (UTC+8)
Publisher:	Elsevier
Abstract:	Sword Brake fern (Pteris ensiformis Burm.) is one of the most common ingredients of traditional herbal drinks in Taiwan. In an effort to identify antioxidants from the aqueous extract of Sword Brake fern (SBF), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity-guided isolation was employed. Three new compounds, kaempferol 3-O-α-l-rhamnopyranoside-7-O-[α-d-apiofuranosyl-(1-2)-β-d-glucopyranoside] (1), 7-O-caffeoylhydroxymaltol 3-O-β-d-glucopyranoside (3) and hispidin 4-O-β-d-glucopyranoside (4), together with five known compounds, kaempferol 3-O-α-l-rhamnopyranosid-7-O-β-d-glucopyranoside (2), caffeic acid (5), 5-O-caffeoylquinic acid (6), 3,5-di-O-caffeoylquinic acid (7) and 4,5-di-O-caffeoylquinic acid (8) were isolated and determined on the basis of spectroscopic analyses. HPLC with UV detector was further employed to analyze the content of each compound in SBF based on the retention time by comparison with isolated pure compounds. It was found that the most abundant phenolic compound was compound 3, followed by compounds 7 and 4. The di-O-caffeoylquinic acids (7 and 8) have the strongest DPPH scavenging potential with IC50 around 10 μM and the highest Trolox equivalent antioxidant capacity (TEAC) about 2 mM. This data indicates that SBF is rich in phenolic antioxidants.
Relation:	Food Chemistry 105(1) ： p.48-56
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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