Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/22185
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    標題: Microwave-assisted acid hydrolysis of toluene diamine conjugates in urine samples for biomarkers in toluene diisocyanate analysis
    作者: Hui-Jung Yeh
    Wei-Chao Lin
    Shiann-Cherng Sheu
    Tung-Sheng Shih
    Lien-Huei Chen
    Ho-Yuan Chang
    貢獻者: 嬰幼兒保育系
    關鍵字: Toluene diisocyanate
    Toluene diamine
    Microwave-assisted heating
    Hydrolysis
    日期: 2007-06
    上傳時間: 2010-01-11 14:08:07 (UTC+8)
    摘要: Toluene diamines (TDAs) in urine have been used widely to determine the amount of toluene diisocyanate (TDI) absorbed by humans. Conventional hydrolysis to prepare a sample of urine takes approximately 16 h. An attempt is made to apply microwave-assisted heating (MAH) to reduce the duration of analysis. Urine collected from rats exposed to a mixture of 2,4- and 2,6-TDI was diluted with non-exposed human urine 1/1250-, 1/500- and 1/250-fold. The urine samples were hydrolyzed by both conventional heating and MAH. The hydrolysis efficiency obtained using MAH significantly exceeded that obtained using conventional heating. Hydrolysis by MAH required only 20 min, 48 times faster than with conventional heating. The use of the MAH method in hydrolysis was demonstrated to be reproducible, timesaving and efficient technique in measuring the concentration of urinary TDAs.
    關聯: Talanta 72(6) : p.1527-1532
    显示于类别:[嬰幼兒保育系] 期刊論文

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