Varing phospholipids were used to prepare liposomes which were used to perform the evaluation in trascorneal characteristics. Enoxacin was selected as a model drug incorporated in various liposomal formulations as a therapeutic dosage form. Using a combination of the ethanol injection technique and freeze-drying process,the encapsulation efficiency became 9-fold greater than that odtained by using a conventional film method. In the in vitro corneal perfusion studies,enoxacin loaded-liposome was transfered through cornea at aslower rate than free drug. However, enoxacin loaded-liposomes were accumulated primarily in the cornea. The increase in the drug corneal retention for the lipids conformed to the following order:DSPC>DPPC.DMPC. Finally. the DSC analysis was utilized to determine the interaction occuring between liposomes and cornea. The liposomes must be transferred into cornea to alter the constitute of cells and can be prodadly taken up by the cornea via endocytosis.