Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate

doi:10.1016/j.jep.2008.08.011

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/21570

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/21570

Title:	Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate
Authors:	Mei-Chun Kuo Chien-Yu Chang Tso-Lin Cheng Ming-JiuanWua
Contributors:	生物科技系
Keywords:	Antrodia camphorata TNF-α IL-6 Phagocytosis CD11b Adenosine
Date:	2008-11
Issue Date:	2009-07-21 14:06:54 (UTC+8)
Publisher:	Elsevier
Abstract:	Aim of the study: Antrodia camphorata, a precious folkloric medicinal mushroom, has been used to treat tumorigenic diseases in Taiwan. This study was to investigate the innate immunity augmentation effects of different fractions prepared from hot water extracts of submerged cultured Antrodia camphorata (AC). Materials and methods: The cytokine induction potency of AC fraction in diluted peripheral blood culture was measured by ELISA. The effects of AC fraction on phagocytic activity and CD11b expressionwere measured by the ingestion of FITC-labeled Escherichia coli and by labeling with PE-labeled CD11b monoclonal antibody, respectively, using flow cytometry. The molecular mass of hot water-soluble polysaccharides and content of adenosine in AC fraction were determined by gel permeation chromatography (GPC) and HPLC, respectively. Results: Themycelia fraction, Fr.MII, and culture filtrate fractions, Fr. E II and Fr. E III, showed the strongest TNF- and IL-6 induction effect as a function of their concentration. These fractions (20 g/ml) also showed marked activity in enhancing phagocytosis in human polymorphonuclear neutrophils (PMN) and monocytes. In parallel, the expression of CD11b, an early marker of PMN activation,was also up-regulated dose-dependently. Composition analysis suggested that immunomodulatory effect of mycelia is mainly attributed to the 10–20 kDa polysaccharides and adenosine. Conclusions: These results provide evidences that Antrodia camphorata can modulate innate immunity and may serve as an adjuvant for tumor treatment.
Relation:	Journal of ethnopharmacology 120(2)：p.196-203
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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