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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/21570


    標題: Immunomodulatory effect of Antrodia camphorata mycelia and culture filtrate
    作者: Mei-Chun Kuo
    Chien-Yu Chang
    Tso-Lin Cheng
    Ming-JiuanWua
    貢獻者: 生物科技系
    關鍵字: Antrodia camphorata
    TNF-α
    IL-6
    Phagocytosis
    CD11b
    Adenosine
    日期: 2008-11
    上傳時間: 2009-07-21 14:06:54 (UTC+8)
    出版者: Elsevier
    摘要: Aim of the study: Antrodia camphorata, a precious folkloric medicinal mushroom, has been used to treat tumorigenic diseases in Taiwan. This study was to investigate the innate immunity augmentation effects of different fractions prepared from hot water extracts of submerged cultured Antrodia camphorata (AC). Materials and methods: The cytokine induction potency of AC fraction in diluted peripheral blood culture was measured by ELISA. The effects of AC fraction on phagocytic activity and CD11b expressionwere measured by the ingestion of FITC-labeled Escherichia coli and by labeling with PE-labeled CD11b monoclonal antibody, respectively, using flow cytometry. The molecular mass of hot water-soluble polysaccharides and content of adenosine in AC fraction were determined by gel permeation chromatography (GPC) and HPLC, respectively.
    Results: Themycelia fraction, Fr.MII, and culture filtrate fractions, Fr. E II and Fr. E III, showed the strongest TNF- and IL-6 induction effect as a function of their concentration. These fractions (20 g/ml) also showed marked activity in enhancing phagocytosis in human polymorphonuclear neutrophils (PMN) and monocytes. In parallel, the expression of CD11b, an early marker of PMN activation,was also up-regulated dose-dependently. Composition analysis suggested that immunomodulatory effect of mycelia is mainly attributed to the 10–20 kDa polysaccharides and adenosine. Conclusions: These results provide evidences that Antrodia camphorata can modulate innate immunity and may serve as an adjuvant for tumor treatment.
    Appears in Collections:[生物科技系(所)] 期刊論文

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