Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/21569
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    標題: Identification of Antioxidants from Rhizome of Davallia solida
    作者: Yung-Husan Chen
    Fang-Rong Chang
    Yih-Jer Lin
    Pei-Wen Hsieh
    Ming-Jiuan Wu
    Yang-Chang Wua
    貢獻者: 生物科技系
    關鍵字: Davallia solida
    Trolox equivalent antioxidant capacity (TEAC)
    日期: 2008-03
    上傳時間: 2009-07-21 14:06:51 (UTC+8)
    出版者: Elsevier
    摘要: Davallia solida rhizome has long been used as an herb tonic to treat osteoporosis, arthralgia, and arthritis. The aqueous extract of D. solida rhizome contains a high content of phenolic compounds [210.8 +/- 4.6 mg catechin equivalents (CE)/g dry weight] and shows a strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (IC50 15.93 +/- 1.21 mu g dry weight/ml). Further solvent partition of the aqueous extract yielded chloroform, n-butanol, and water layers. Among them, n-butanol layer has the highest phenol content (806.3 +/- 12.3 mu g CE/g dry weight) and DPPH scavenging potential (IC50 = 3.93 +/- 0.31 mu g dry weight/ml). Isolation and purification from the n-butanol layer identified 12 compounds. They included four new compounds: 3'-O-p-hydroxybenzoylmangiferin (1), 4'-O-p-hydroxybenzoylmangiferin (2), 6'-O-p-hydroxybenzoylmangiferin (3), and 3-O-p-hydroxybenzoylmangiferin (4); as well as eight known compounds: mangiferin (5), 2-C-beta-D-xylopyranosyl-1,3,6,7-tetrahydroxyxanthone (6), 4 beta-carboxymethyl-(-)-epicatechin (7), 4 beta-carboxymethyl-(-)-epicatechin methyl ester (8), eriodictyol (9), eriodictyol-8-C-beta-D-glucopyranoside (10), icariside E-5 (11), and icariside E-3 (12). DPPH scavenging and Trolox equivalent antioxidant capacity (TEAC) analyses revealed that the most potent antioxidants are 1, 2, and 3, which exerted more than triple activity as compared with the positive controls, alpha-tocopherol and Trolox. (C) 2007 Elsevier Ltd. All rights reserved.
    關聯: Food chemistry 107(2):p.684-691
    显示于类别:[生物科技系(所)] 期刊論文


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