霍亂毒素單株及多株抗體之生產

CNU IR > Pharmacy and Science > Dept. of Food Science & Technology > Chna Project > Item 310902800/1126

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Title:	霍亂毒素單株及多株抗體之生產 Production and Purification of New Monoclonal and Polyclonal Antibodies Against Cholera Toxin
Authors:	周淑芬
Contributors:	食品科技系
Date:	2003
Issue Date:	2008-06-26 15:06:54 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學食品科技系
Abstract:	The aim of this study was to produce monoclonal and polyclonal antibodies against cholera toxin (CT). Hyperimmune ICR mice produced polyclonal antibodies after injection with 0.5 ml pristane, and were injected with NS-1 myeloma cells two weeks later. Hyperimmune Balb/c mice were used for the production of monoclonal antibodies (MAbs). After these mice were immunized four times and given a final boost, their spleen cells were collected and fused with NS-1 myeloma cells under the presence of PEG 1500. The fused cells were then selected in the hypoxathine, aminopterine, and thymidine (HAT)-RPMIX medium. Anti-CT antibody-secreting hybridoma cell lines with high titer were cloned by enzyme-linked immunosorbent assay (ELISA) and then subcloned by limiting dilution in 15% fetal bovine serum (FBS) HT-RPMIX medium. Eleven murine hybridoma producing anti-CT MAbs were obtained and designated CT-A2, CT-B4, CT-B11, CT-C7, CT-D7, CT-E8, CT-F4, CT-F2, CT-F8, CT-E3, CT-E6. Isotypes of these MAbs were identified as IgG1 heavy chain and κ light chain. Hitrap Protein A column was used for the purification of polyclonal and monoclonal antibodies.
Relation:	計畫編號：CNFH9204
Appears in Collections:	[Dept. of Food Science & Technology] Chna Project

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