此方法的開發能快速、有效的偵測染料含量，可作為檢驗市面產品是否超過限制含量的一種方法。 According to the convenience, and the efficiency, and environmental protection concept, this research applies the UV-VIS Spectrophotometers analysis method to examine the element of the hair dye in order to achieve detective effect quickly and accurately . The purpose is to discuss the effect of the size of molecular weight that is resulted from permanent hair dye oxidizing reaction on the quantities percutaneous absorption .
Using p-Phenylenediamine as the dye precursor, 6-Amino-o-cresol, and 1-Napthol as the Secondary intermediates, the hydrogen peroxide as the oxidant to proceed the oxidizing reaction, that makes the different compounds of benzene ring molecular weight be synthesized.Through thin layer chromagraphy (TLC) purification and 1H- Nuclear magnetic resonance(1HNMR) to authenticate the structural formula, and using pigskin as experimental materials to proceed percutaneous absorption experiment to obtain the value and concentration of light absorption of donor compartment decrement, receptor compartment increment , and extract immersion pigskin .
The result shows that the quantity of percutaneous absorption of the small molecular weight of dye precursor and Secondary intermediates will be increased with time . The compound of big molecular weight has less percutaneous absorption quantity than the small , and therefore the size of molecular weight will affect the percutaneous absorption quantity.
This method could be developed to detect the contents of hair dye ingredient effectively and fast, and could be one of test methods to examine which products exceed the regulation quantity of the contents on the market.